mouse embryonic fibroblast nih 3t3 cells  (ATCC)

Journal: Materials Today Bio

Article Title: Feasibility of combining JAK1 gene editing via CRISPR-CasRx with EGCG–lactoferrin nanoparticle therapy in a microneedle-based platform for atopic dermatitis

doi: 10.1016/j.mtbio.2026.102884

Figure Lengend Snippet: Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in NIH-3T3 and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).

Article Snippet: Mouse embryonic fibroblast NIH/3T3 cells and mouse dendritic DC2.4 cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Plasmid Preparation, Zeta Potential Analyzer, Agarose Gel Electrophoresis, Transmission Assay, Electron Microscopy, Fluorescence, Transfection, Expressing

Journal: Animal Bioscience

Article Title: Bulbophyllum drymoglossum aqueous extract modulates immunometabolism and oxidative stress in porcine alveolar macrophages

doi: 10.5713/ab.25.0638

Figure Lengend Snippet: Effects of Bulbophyllum drymoglossum aqueous extract (BDAE) on cell viability, proliferation, and chemical composition. (A) Representative images of Bulbophyllum drymoglossum leaves and aqueous extract (BDAE) preparation by high-temperature extraction (110°C, 15 min). (B) Relative viability of NIH/3T3 fibroblasts and porcine alveolar macrophages (3D4/31) after 24 h exposure to increasing concentrations of BDAE, measured using the WST-8 assay. ns, not significant; **** p<0.0001 versus vehicle control. (C) Effects of BDAE on cell proliferation in 3D4/31 cells during 3 days of culture with indicated extract concentrations. (D) Representative GC–MS total ion chromatogram of BDAE, showing major metabolite peaks. (E) Table of major compounds in BDAE as identified by GC-MS, including retention time, area percentage, compound names, similarity index (SI), and molecular formula. GC-MS, gas chromatography–mass spectrometry.

Article Snippet: Porcine alveolar macrophages (3D4/31; ATCC CRL-2844) and mouse embryonic fibroblasts (NIH3T3; ATCC CRL-1658) were maintained at 37°C in a 5% CO 2 humidified incubator.

Techniques: Extraction, Control, Gas Chromatography-Mass Spectrometry, Gas Chromatography, Mass Spectrometry